Changes of circulating hematopoietic progenitor cells in patients undergoing coronary artery bypass graft surgery.
- Author:
Lin ZHANG
1
;
Changqing GAO
;
Rong WANG
;
Cangsong XIAO
;
Yang WU
Author Information
- Publication Type:Journal Article
- MeSH: Coronary Artery Bypass; Coronary Artery Bypass, Off-Pump; Hematopoietic Stem Cells; cytology; Humans; Postoperative Period
- From: Journal of Southern Medical University 2014;34(11):1574-1577
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the changes of circulating hematopoietic progenitor cells (cHPCs) in patients undergoing coronary artery bypass graft (CABG) surgery.
METHODSFifty-one patients scheduled to undergo elective CABG surgery were prospectively enrolled in this study. Blood samples were collected at baseline and at 1, 3, and 5 days after the surgery. CD34⁺ CD45dim, CD133⁺ CD45dim, and CD34⁺ CD133⁺ CD45dim cells were measured by flow cytometry. The functionality of the circulating progenitor cells were studied in vitro using clonogenic and migration assays.
RESULTSAll the elective patients showed a significant postoperative increase of CD34⁺ CD45dim (1.51 ± 1.26 vs 2.02 ± 1.16, P=0.036) and CD133⁺ CD45dim cells (1.16 ± 0.85 vs 1.65 ± 0.99, P=0.008) irrespective of on-pump or off-pump CABG. The perioperative changes of the cHPCs was more significant in patients undergoing on-pump surgery than those having off-pump surgery.
CONCLUSIONIn patients undergoing elective cardiac surgery, cHPC level increased early after the surgery but recovers the normal level over time.
