Preliminary screening of downstream proteins of Sox2 and role of Sox2 in colonic cancer cell migration and invasion.
- Author:
Min ZHOU
1
,
2
;
Yanxia LU
;
Li YUAN
;
Lin ZHENG
;
Yan LIU
;
Min HONG
;
Chao ZHANG
;
Xuenong LI
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Cell Movement; Cell Proliferation; Colonic Neoplasms; metabolism; Down-Regulation; Humans; Proteomics; Ribosomal Proteins; metabolism; SOXB1 Transcription Factors; metabolism; Up-Regulation
- From: Journal of Southern Medical University 2014;34(11):1594-1600
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen the down-stream proteins of transcription factor Sox2 and explore the role of Sox2 in the proliferation and migration of colonic cancer cells in vitro.
METHODSThe cellular proteins were separated by SDS-PAGE electrophoresis and stained with Coomassie blue and amine plated silver. The differentially expressed proteins was identified by mass spectrometry and verified by QPCR and Western blotting. A cell counting kit-8 (CCK8) assay was performed to evaluate the cell proliferation, and the cell migration was assessed using Transwell assay.
RESULTSS3a was identified by proteomics technology as a Sox2-downregulated protein while ENO1 and gama-actin the up-regulated proteins. QPCR and Western blotting analyses showed that overexpression of Sox2 significantly decreased the expression of S3a (P<0.005) and increased the expression of ENO1(P<0.05), but had no significant effect on gama-actin expression. Sox2 overexpression obviously promoted cell proliferation and migration (P<0.05), while inhibition of Sox2 produced contrary effects (P<0.05).
CONCLUSIONSox2 negatively regulates S3a expression and positively regulates ENO1 expression to promot the proliferation and migration of colonic cancer cells.
