Effect of acute exposure to high altitude on pharmacokinetics of propranolol and metoprolol in rats.
- Author:
Juanhong ZHANG
1
;
Rong WANG
;
Hua XIE
;
Qiang YIN
;
Zhengping JIA
;
Wenbin LI
Author Information
- Publication Type:Journal Article
- MeSH: Altitude; Animals; Chromatography, Liquid; Metoprolol; pharmacokinetics; Propranolol; pharmacokinetics; Protein Binding; Rats; Rats, Wistar; Tandem Mass Spectrometry
- From: Journal of Southern Medical University 2014;34(11):1616-1620
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the pharmacokinetics of propranolol and metoprolol in rats after acute exposure to high altitude.
METHODSWistar rats were randomly assigned into 4 groups for treatment with intragastric administration of propranolol or metoprolol after acute exposure to high altitude (4010 m) or normal altitude (50 m). Venous blood samples were collected from the rats at different time points after drug administration to determine the drug concentrations in the plasma and plasma ultrafiltrate using liquid chromatography-mass spectrometry (LC-MS/MS).
RESULTSThe protein binding rate of propranolol was significantly increased but that of metoprolol remained unchanged after acute exposure to high altitude. Compared with the rats exposed to normal altitude, the rats with acute exposure to high altitude showed significant alterations in the pharmacokinetic parameters of the drugs, shown by increased Cmax and AUC, prolonged t1/2 and MRT, and lowered Clz/F of propranolol, and by increased Tmax and prolonged t1/2 and MRT of metoprolol without obvious changes of the parameters of the compartmental model.
CONCLUSIONSignificant changes in the pharmacokinetics of propranolol and metoprolol occur in rats after acute exposure to high altitude possibly in relation to, apart from the changes in plasma protein binding ratio and blood gas, alterations in metabolic enzyme activities, increased blood viscosity, and species and general conditions of the animals.