Live cell fluorescent imaging and cytotoxicity assessment of pH fluorescent probe based on styrylcyanine dyes.
- Author:
Lixia HUANG
1
;
Xiang LIU
;
Danting CUI
;
Yikai XU
;
Wenwei YOU
;
Yichen YAN
;
Xinwei LU
;
Ruiyuan LIU
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Fluorescence; Fluorescent Dyes; Hydrogen-Ion Concentration; Optical Imaging
- From: Journal of Southern Medical University 2014;34(11):1642-1645
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo prepare a pH fluorescence probe based on styrylcyanine dyes for live cell imaging.
METHODSThe Probe 1 was prepared by reaction of 4-pyridinecarboxaldehyde with 1,1,2-trimethylbenz[e]indole. The influence of pH on the fluorescent properties was examined, and the cell viability was examined using cell counting kit-8. The Probe 1 was used as a pH fluorescence probe in living cell.
RESULTSProbe 1 emitted green fluorescence under neutral and basic conditions but orange fluorescence under acid condition. Probe 1 selectively stained the cytoplasmic regions of living cells without significantly affecting the cell viability.
CONCLUSIONThe pH-sensitive fluorescent probe prepared based on styrylcyanine possesses good ability of cell membrane permeation for live cell fluorescent imaging.
