Establishment of a method for detecting peripheral blood circulating brain microvascular endothelial cells, a novel biomarker for blood-brain barrier injury.
- Author:
Yan LI
1
;
Lei DU
;
Lin YUAN
;
Dexi CHEN
;
Jiawen QIU
;
Xiaolong HE
;
Hong CAO
;
Shenghe HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Acquired Immunodeficiency Syndrome; physiopathology; Biomarkers; Blood-Brain Barrier; pathology; Cell Separation; methods; Cells, Cultured; Endothelial Progenitor Cells; cytology; Humans
- From: Journal of Southern Medical University 2014;34(12):1733-1737
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for detecting circulating brain microvascular endothelial cells (cBMECs), a novel biomarker of blood-brain barrier (BBB) injury.
METHODSBlood samples were collected from 33 patients with AIDS encephalitis and 13 healthy subjects for detection of cBMECs, cECs and EPCs using magnetic affinity isolation and immune identification technology.
RESULTSThe numbers of cBMECs, cECs and EPCs were significantly higher in the AIDS patients than in the control subjects (t=4.298, P<0.01; t=4.886, P<0.01; t=4.889, P<0.01). An significant association was also noted between HIV load and cBMEC number (r=0.928, P<0.01).
CONCLUSIONWe have successfully established a method for detecting peripheral blood cBMECs, which can be of important value in non-invasive assessment of BBB injury.
