Alternative polyadenylation site analysis of tumor-related genes based on 3'RACE in gastric cancer cells.

Author: Dengpan LAI ¹ ; Jian CHEN ; Yani KANG
Author Information

1. Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine of Ministry of Education, Shanghai Jiaotong University, Shanghai 200240, China.E-mail: dengpanlai@gmail.com.
Publication Type:Journal Article
MeSH: Cell Line, Tumor; Cell Transformation, Neoplastic; Genes, Neoplasm; Humans; Polyadenylation; Stomach Neoplasms; genetics
From: Journal of Southern Medical University 2014;34(12):1738-1742
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo analyze the alteration in alternative polyadenylation (APA) sites of tumor-related genes in gastric cancer cells.
METHODSWe used 3'RACE to capture the APA sites of two tumor-related genes (HSP90α and SEC11A) in gastric cancer cell lines MKN45, MKN28 and AGS, and compared the results with annotated poly(A) sites in UCSC database.
RESULTSWe found new APA sites in the two tumor-related genes in gastric cancer cells to produce new mRNA isoforms with different 3'UTRs.
CONCLUSIONSThere are new mRNA isoforms of HSP90α and SEC11A derived from ATA in gastric cancer cells, which provides new insights into the mechanisms of gastric tumorigenesis.