Lentivirus-mediated shRNA interference of Cx26 suppresses epithelial mesenchymal transition and invasion of highly invasive hepatocellular carcinoma cells in vitro.
- Author:
Jie YANG
1
;
Guihui QIN
;
Junze CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Cadherins; metabolism; Carcinoma, Hepatocellular; pathology; Cell Line, Tumor; Connexin 26; Connexins; genetics; metabolism; Down-Regulation; Epithelial-Mesenchymal Transition; Genetic Vectors; Humans; Lentivirus; Liver Neoplasms; pathology; RNA, Messenger; RNA, Small Interfering; Real-Time Polymerase Chain Reaction; Transfection; Vimentin; metabolism
- From: Journal of Southern Medical University 2014;34(12):1743-1747
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of lentivirus-mediated shRNA interference of Cx26 on epithelial-mesenchymal transition (EMT) and invasion of highly invasive human hepatocellular carcinoma cells in vitro.
METHODSSK-Hep-1 cells were infected with the lentivirus for delivering Cx26 shRNA, and the stably transfected cells were selected by puromycin. The interference efficiency of shRNA-Cx26 was assessed with real-time PCR and Western blotting. The morphological changes of the transfected SK-Hep-1 cells were observed microscopically, and the protein expressions of E-cadherin and vimentin were detected using Western blotting. The effect of Cx26 interference on the invasiveness of SK-Hep-1 cells was determined by Transwell invasion assay.
RESULTSCompared with SK-Hep-1 cells infected with empty EGFP vector and uninfected cells, the cells transfected with shRNA-Cx26 showed significantly reduced mRNA and protein expressions of Cx26 (P<0.01), which resulted in obvious morphological conversion from mesenchymal cells to epithelial cells. shRNA-Cx26-transfected cells showed significantly increased E-cadherin protein expression (P<0.01) but decreased vimentin expression (P<0.01) with obviously attenuated invasive ability in vitro (P<0.01).
CONCLUSIONTargeted down-regulation of Cx26 expression can inhibit the EMT and invasion of SK-Hep-1 cells in vitro.