Effects of Biejiajian Pills on Wnt signal pathway signal molecules β-catenin/TCF4 complex activities and downstream proteins cyclin D1 and MMP-2 in hepatocellular carcinoma cells.
- Author:
Bin WEN
1
;
Haitao SUN
;
Songqi HE
;
Yang CHENG
;
Wenyan JIA
;
Eryan FAN
;
Jie PANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; metabolism; Carcinoma, Hepatocellular; metabolism; Cyclin D1; metabolism; Drugs, Chinese Herbal; pharmacology; Hep G2 Cells; Humans; Liver Neoplasms; metabolism; Matrix Metalloproteinase 2; metabolism; Rats; Transcription Factor 4; Transcription Factors; metabolism; Wnt Proteins; Wnt Signaling Pathway; beta Catenin; metabolism
- From: Journal of Southern Medical University 2014;34(12):1758-1762
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of Biejiajian Pills on Wnt signal pathway and the mechanisms underlying its action to suppress the invasiveness of hepatocellular carcinoma.
METHODSHepG2 cells cultured in the serum of rats fed with Biejiajian Pills for 48 h were examined for β-catenin expression using immunofluorescence, β-catenin/TCF4 complex activity with luciferase, and expressions of the downstream proteins cyclin D1 and MMP-2 using qRT-PCR.
RESULTSBiejiajian Pills-treated sera significantly reduced the expressions of cytoplasmic and nuclear β-catenin protein, cyclin D1 and MMP-2 proteins and lowered the activities of β-catenin/TCF4 complex.
CONCLUSIONBiejiajian Pills may serve as a potential anti-tumor agent, whose effect might be mediated by inhibiting the Wnt/β-catenin pathway.
