Expression of chemokine receptor CXCR7 in gastric cancer tissues and cell lines.
- Author:
Ameng SHI
1
;
Lei DONG
;
Haitao SHI
;
Miao JIA
;
Xiaoyan GUO
;
Jiong JIANG
;
Bin QIN
Author Information
- Publication Type:Journal Article
- MeSH: Cell Differentiation; Cell Line, Tumor; Disease Progression; Helicobacter Infections; Humans; Immunohistochemistry; Receptors, CXCR; metabolism; Signal Transduction; Stomach Neoplasms; diagnosis; metabolism
- From: Journal of Southern Medical University 2014;34(12):1780-1784
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression status of CXCR7 in gastric cancer tissues and cell lines.
METHODSThe expression status of CXCR7 was detected in 35 primary gastric cancer tissues and matched adjacent tissues by immunohistochemistry and RT-PCR. The correlation of CXCR7 expression with the clinicopathological parameters and risk factors of gastric cancer was analyzed. The expression of CXCR7 in gastric cell lines (HGC-27, MGC-803, BGC-823, SGC-7901 and MKN-28) was also detected by immunofluorescence assay.
RESULTSThe expression of CXCR7 was significantly higher in gastric cancer tissues than in adjacent tissues (P<0.01). CXCR7 expression was not correlated with age, gender, smoking history, Helicobacter pylori infection, tumor location or the pathological type, but showed a higher expression level in patients with a alcohol-drinking history than in those without (P<0.05). CXCR7 was expressed with variable intensities in the 5 gastric cancer cell lines without correlation with the degrees of cell differentiation; its expression was the highest in SGC-7901 cells, a moderately differentiated human gastric adenocarcinoma cell line.
CONCLUSIONSCXCR7 is highly expressed in gastric cancer tissues with variable intensities in 5 gastric cancer cell lines, suggesting its important role in gastric cancer progression.
