Comparison of the expression profiles of cell death factors in articular cartilage between Kashin-Beck disease and osteoarthritis.
- Author:
Shixun WU
1
;
Xiong GUO
;
Feng ZHANG
;
Jingjing ZHENG
;
Zengtie ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Apoptosis Regulatory Proteins; metabolism; Cartilage, Articular; metabolism; pathology; Chondrocytes; metabolism; Early Growth Response Protein 1; metabolism; Humans; Immunohistochemistry; Kashin-Beck Disease; metabolism; Neoplasm Proteins; metabolism; Osteoarthritis; metabolism; Transcriptome
- From: Journal of Southern Medical University 2014;34(12):1785-1789
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the expressions of programmed cell death 5 (PDCD5) and early growth response protein-1 (EGR-1) in the articular cartilage between Kashin-Beck disease (KBD) and primary osteoarthritis and the roles of these factors in KBD cartilage.
METHODSCartilage specimens were collected from 10 confirmed KBD patients, 15 osteoarthritic patients and 6 healthy subjects. The expression levels of PDCD5 and EGR-1 in the cartilage were detected by immunohistochemistry staining, and the positive chondrocyte counts were recorded in the different layers of KBD and OA cartilages.
RESULTSThe KBD cartilages contained a significantly higher percentage of PDCD5-positive chondrocytes in the middle layer [(41.35 ± 2.97)%] than OA cartilages [(26.48 ± 2.04)%, P=0.001] and normal cartilages [(19.02 ± 1.88)%, P=0.000] with also obvious PDCD5 over-expression in the deeper layer compared to OA (P=0.000) and normal cartilages (P=0.029), but PDCD5 expression in the superficial layer of the cartilages showed no significant difference among the 3 groups(P>0.05). The average EGR-1 positivity rate in the superficial layer of the cartilage was significantly higher in KBD patients than in OA patients (P=0.000) and healthy controls (P=0.000), but in the middle layer, its positivity rate in KBD patients was higher than that in the normal control (P=0.017) but lower than that of OA cartilage (P=0.002); EGR-1 expression in the deeper layer was comparable in KBD and OA cartilages but both was higher than that in normal cartilages. PDCD5 and EGR-1 expressions were not correlated in either KBD or normal cartilages, but were positively correlated in the superficial layer of OA cartilages.
CONCLUSIONSKBD cartilages show a significantly increased PDCD5 expression in the deeper layer and enhanced EGR-1 expression in both superficial and deeper layers, suggesting the involvement of PDCD5 and EGR-1 in the pathogenesis of KBD.