An experimental study on the expression of heme oxygenase-2 mRNA in Hirschsprung's disease.
- Author:
Min ZHU
1
;
Mingfa WEI
;
Fang LIU
Author Information
1. Department of Pediatric Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
- Publication Type:Journal Article
- MeSH:
Child;
Child, Preschool;
Colon;
enzymology;
Female;
Heme Oxygenase (Decyclizing);
biosynthesis;
genetics;
Hirschsprung Disease;
enzymology;
etiology;
genetics;
Humans;
Infant;
Infant, Newborn;
Male;
RNA, Messenger;
biosynthesis;
genetics
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2002;22(2):152-154
- CountryChina
- Language:English
-
Abstract:
In order to investigate the relationship between the expression of heme oxygenase-2 (HO-2) mRNA and the pathogenesis of Hirschsprung's disease (HD), total ribonucleic acid (RNA) was extracted in the aganglionic and ganglionic segments of colon respectively from 15 cases of HD. The single-stranded cDNA of HO-2 was synthesized and further amplified by reverse transcription-polymerase chain reaction (RT-PCR). The expression of HO-2 mRNA was normal in ganglionic segments, but absent in aganglionic segments. It is concluded that the absence of HO-2 mRNA expression may be an important mechanism responsible for HD.