Experimental study of plasmid TGF-beta 1 DNA gene transfer with lipofectamine into rabbit corneal epithelial cells in vitro.
- Author:
Qiong HUANG
1
;
Yanhua HU
;
Fagang JIANG
;
Hong CHEN
Author Information
1. Department of Ophthalmology, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Division;
Cells, Cultured;
Epithelium, Corneal;
cytology;
metabolism;
Gene Transfer Techniques;
Genetic Vectors;
Lipids;
pharmacology;
Plasmids;
genetics;
Rabbits;
Transforming Growth Factor beta;
biosynthesis;
genetics;
Transforming Growth Factor beta1
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2002;22(1):62-65
- CountryChina
- Language:English
-
Abstract:
To investigate whether the TGF-beta 1 plasmid DNA carried by lipofectamine could be introduced into cultured rabbit corneal epithelial cells, specific expression of the plasmid pMAM TGF-beta 1 in the cultured corneal epithelial cells was studied. Two days after 12 h of transfection of pMAMT-GF-beta 1 mediated by lipofectamine into the cultured corneal epithelial cells, the TGF-beta 1 protein expression specific for pMAMTGF-beta 1 in the cells was detected by means of immunohistochemical staining and the positive rate was 23.37%. The results suggested that foreign plasmid DNA could be effectively delivered into cultured rabbit corneal epithelial cells by means of lipofectamine, and this will provide a promising method of studying TGF-beta 1 on the mechanism of physiology and pathology concerned with corneal epithelial cells.
