Study on the mechanism of oocyte aneuploidy formation by multicolor fluorescence in situ hybridization.

Author: Wen CHEN ¹ ; Liselotte METTLER
Author Information

1. Department of Gynecology and Obstetrics, Tongji Hospital of Tongji Medical College, Huazhong Science and Technology University, Wuhan, Hubei, 430030 PR China. chenwenyyy@yahoo.com
Publication Type:Journal Article
MeSH: Adult; Aneuploidy; Female; Humans; In Situ Hybridization, Fluorescence; methods; Karyotyping; Oocytes; ultrastructure
From: Chinese Journal of Medical Genetics 2004;21(3):203-206
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the mechanism of oocyte aneuploidy formation.
METHODSThe unfertilized oocytes were fixed 1-3 days after oocyte retrieval. Multicolor fluorescence in situ hybridization (M-FISH) was performed according to the Vysis protocol to check the chromosome status in oocytes by using centromeric enumerator probes for chromosomes 16, 18 and locus-specific probes for chromosomes 13, 21 and 22.
RESULTS47% oocytes were found to be normal, while 53 % oocytes were abnormal. Nondisjunction was found in 22(18%) oocytes, unbalanced predivision of chromatids in 15(12%) oocytes, balanced predivision of chromatids in 45(36%) oocytes. The balanced predivision rate in oocytes aged in vitro>24h was much higher than that in oocytes aged in vitro
CONCLUSIONBoth nondisjunction, balanced and unbalanced predivision of chromatids are involved in the oocyte aneuploidy formation. Balanced predivision is related to the time in culture.