Polymorphism and multiplex amplification of 3 X-chromosome specific short tandem repeat loci.
- Author:
Qiu-ling LIU
1
;
De-jian LÜ
;
Wei CUI
Author Information
- Publication Type:Journal Article
- MeSH: Chromosome Mapping; Chromosomes, Human, X; Female; Gene Amplification; Haplotypes; Humans; Male; Paternity; Polymorphism, Genetic; Tandem Repeat Sequences
- From: Chinese Journal of Medical Genetics 2004;21(3):233-235
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo devise a multiplex PCR system of three X-chromosome specific short tandem repeat (X-STR) loci and study the genetic polymorphism.
METHODSDXS6799, DXS6804 and DXS6854 were amplified simultaneously using a multiplex system and were typed by polyacrylamide gel electrophoresis and silver staining.
RESULTSA total of 262 male and 255 female individuals from Guangdong Han population were tested; each locus showed 7 alleles. 73 haplotypes were detected in the male individuals. The haplotype diversity reached 0.9674.
CONCLUSIONThe 3 X-STR multiplex system is relatively abundant in polymorphic information for forensic identification and paternity testing.
