Microsatellite instability and loss of heterozygosity of human leucocyte antigen class I gene in cervical carcinoma.
- Author:
Yuan RONG
1
;
Xu-feng WU
;
Guan-min CHEN
;
Min ZHAO
;
Xin-xing WU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Female; Histocompatibility Antigens Class I; genetics; Humans; Loss of Heterozygosity; genetics; Microsatellite Instability; Middle Aged; Polymerase Chain Reaction; Uterine Cervical Neoplasms; genetics
- From: Chinese Journal of Medical Genetics 2004;21(4):342-346
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the frequency of microsatellite instability (MSI) and loss of heterozygosity (LOH) at human leucocyte antigen(HLA) class I loci in cervical carcinoma and the detailed deletion mapping in this region.
METHODSLOH and MSI of HLA class I genes were analyzed in 30 paired blood and tumor samples by PCR based single-stranded length polymorphism (PCR-SSLP).
RESULTSOf the 30 cases, 23(76.7%) showed LOH at one or more loci. Higher frequencies of LOH were found at four loci: C3_2_11 (50%), C1_4_4 (37%), C1_2_5 (36.7%), D6S276 (48.3%). MSI was found in 20 out of 30 cases (66.7%).
CONCLUSIONThe data suggest that the LOH and MSI of HLA class I gene might participate in the carcinogenesis of cervical carcinoma. Meanwhile, the minimal deletion region might be defined between C1_2_5 and C3_2_11, thus providing the evidence for cloning the tumor suppressor genes associated with cervical carcinoma.
