The associations of HLA-DQB1 gene with onset age and autoantibodies in type 1 diabetes.
- Author:
Chang-li LIU
1
;
Ye-rong YU
;
Hong LIU
;
Xiang-xun ZHANG
;
Gui-zhi ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Age of Onset; Autoantibodies; immunology; Child; Child, Preschool; China; epidemiology; Diabetes Mellitus, Type 1; epidemiology; genetics; immunology; Enzyme-Linked Immunosorbent Assay; Female; Genetic Predisposition to Disease; genetics; Glutamate Decarboxylase; immunology; HLA-DQ Antigens; genetics; HLA-DQ beta-Chains; Humans; Male; Membrane Glycoproteins; genetics; Middle Aged; Polymerase Chain Reaction; Young Adult
- From: Chinese Journal of Medical Genetics 2004;21(4):368-371
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the associations of human leukocyte antigen (HLA) DQB1 gene with onset age and autoantibodies in type 1 diabetes mellitus(T1DM) in Chinese Han population in Sichuan area.
METHODSForty-six type 1 diabetic patients and 52 healthy control subjects were involved in this study. HLA-DQB1 typing was performed by polymerase chain reaction-sequence specific primer(PCR-SSP). Glutamic acid decarboxylase antibody (GADA) and islet cell antibody (ICA) were qualitatively analyzed by enzyme linked immunosorbent assay (ELISA).
RESULTSThe positive rate of DQB1*0201 was higher in T1DM than in controls (OR=18, P<0.005), but those of DQB1*0601, *0602 were higher in controls than in T1DM(OR=0.07, 0.31 respectively, both P<0.05).The positive rate of DQB1*0602 in type 1 diabetic patients with onset age>or=20 years was higher than that in the patients with onset age <20 years (P<0.05). GADA was more frequent in DQB1*0201(+) patients than in DQB1*0201 (-) patients (P<0.025).
CONCLUSIONThe findings show that DQB1*0201 is susceptible to T1DM, whereas DQB1*0601, *0602 are protective in Chinese Han population in Sichuan area. DQB1*0602 may delay the onset of T1DM. The positive rate of DQB1*0201 correlates positively with that of GADA.
