Differential expression of motility-related protein-1/CD9 gene in gastric cancers and premalignant lesions.
- Author:
Wei-ming ZHANG
1
;
Jie ZHENG
;
Yao XU
;
Wen-tian LIU
;
Yan-yun LI
;
Jun XING
;
Xin GENG
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, CD; genetics; Blotting, Northern; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Humans; Membrane Glycoproteins; genetics; Precancerous Conditions; genetics; Reverse Transcriptase Polymerase Chain Reaction; Stomach Neoplasms; genetics; Tetraspanin-29
- From: Chinese Journal of Medical Genetics 2004;21(5):426-429
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo clone and identify gastric cancer-related genes and explore the possible pathogenic mechanism of gastric cancer.
METHODSThe differentially expressed cDNA bands were isolated by fluorescent mRNA differential display in gastric cancer specimens, matched normal gastric mucosa and premalignant lesions. The motility-related protein-1 (MRP-1/CD9) gene was one of the down-regulated genes. MRP-1/CD9 gene expression in different kinds of gastric tissue was analyzed by Northern blot and reverse transcription-polymerase chain reaction (RT-PCR).
RESULTSMRP-1/CD9 expression was down-regulated in all gastric cancer tissues. Northern blot analysis confirmed this differential expression. RT-PCR analysis showed that the MRP-1/CD9 gene expression was much lower in gastric cancers(0.31+/-0.18) than in the matched normal gastric tissue (0.49+/-0.24) and in the premalignant lesions (0.47+/-0.18) (P<0.05), and its expression in intestinal type gastric cancer (0.38+/-0.16) was higher than that in diffuse type gastric cancer (0.22+/-0.17) (P<0.05).
CONCLUSIONThe MRP-1/CD9 gene expression was down-regulated in gastric cancer, its expression was probably related to the carcinogenesis and histology types of gastric cancer.
