Detecting microdeletions of the Y chromosome in patients with high follicle-stimulating hormone azoospermia.

Author: Li-quan WANG ¹ ; Chen-ming XU ; Fan JIN ; Yu-li QIAN ; He-feng HUANG
Author Information

1. The Affiliated Obstetrics and Gynecology Hospital, College of Medical Sciences, Zhejiang University, Hangzhou, Zhejiang, 310006 P.R.China.
Publication Type:Journal Article
MeSH: Azoospermia; genetics; metabolism; Chromosome Deletion; Chromosomes, Human, Y; genetics; Female; Follicle Stimulating Hormone; metabolism; Humans; Infertility, Male; genetics; metabolism; Male; Polymerase Chain Reaction; Sequence Tagged Sites
From: Chinese Journal of Medical Genetics 2004;21(5):485-487
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo explore the relationship between the patients' high follicle-stimulating hormone (FSH) azoospermia and microdeletions in Y chromosome.
METHODSEleven sequence tagged sites (STSs) in Yq were detected by PCR in 16 male patients' high FSH azoospermia.
RESULTSMicrodeletions were observed in 6 of 16 male patients and the deletion rate was 37.5%(6/16). Five types of microdeletions were detected: AZFc(SY152), AZFc (SY152+SY254)+AZFd (SY153), AZFc (SY152+SY254+SY255)+AZFd (SY153), AZFc (SY152+SY158+SY255)+AZFd (SY153),and AZFb (SY130)+AZFc (SY158+SY254+SY255)+AZFd (SY153) respectively.
CONCLUSIONMicrodeletion of Y chromosome was one of the important reasons of the patients' high FSH azoospermia. Before the application of assisted-reproductive technology (ART) to the patients, it is necessary to detect the microdeletions, especially AZFc and AZFd.