Lycopene attenuates angiotensin Ⅱ induced oxidative stress in H9c2 cells
10.3760/cma.j.issn.0253-3758.2015.04.012
- VernacularTitle:番茄红素可改善血管紧张素Ⅱ诱导的H9c2细胞氧化应激
- Author:
Yuan LIU
1
;
Qizhu TANG
;
Zhefu HU
;
Wei DENG
;
Lina LI
Author Information
1. 430060,武汉大学人民医院心内科
- Keywords:
Myocardium;
Oxidative stress;
Lycopene
- From:
Chinese Journal of Cardiology
2015;43(4):341-346
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Lycopene (Lyc) on Ang Ⅱ induced oxidative stress in H9c2 cell line derived from rat cardiac tissue,and to explore related mechanisms.Methods H9c2 cells were divided into 6 groups:control group,Ang Ⅱ group (1 μmol/L),Ang Ⅱ (1 μmol/L) + low dose Lyc (3.125 nmol/L) group,AngⅡ (1 μmol/L) + moderate dose Lyc(6.25 nmol/L)group and Ang Ⅱ (1 μmol/L) + high dose Lyc(12.5 nmol/L) group and Lyc group (12.5 nnmol/L).Cell growth was determined by CCK8 assay,ROS generation was detected using a Microplate reader and Fluorescence microscopy,the expression of NOX2 was determined by Western blot,mRNA expression of p47phox,SOD1 and SOD2 were determined by Real Time-PCR,MDA was detected by ELISA kit.Results Compared to control group,cell survival was significantly reduced and ROS generation was significantly increased post Ang Ⅱ stimulation,cotreatment with Lyc significantly improved cell survival and reduced ROS generation in a dosedependent manner (all P ＜ 0.01).mRNA expression of SOD1 and SOD2 was significantly downregulated while MDA concentration was significantly increased in Ang Ⅱ treated cells,which could be significantly reversed by cotreatment with Lyc in a dose dependent manner (all P ＜ 0.01).Protein expression of NOX2 and mRNA expression of p47phox were significantly upregulated post Ang Ⅱ and which could be significantly downregulated by cotreatment with Lyc in a dose-dependent manner (all P ＜ 0.01).Conclusion Lyc could attenuate Ang Ⅱ induced oxidative stress and this effect is linked with its capacity of reducing ROS generation and enhancing cellular ROS scavenging ability in H9c2 cells.
