Liraglutide attenuates myocardial ischemia/reperfusion injury possibly through reducing cardiomyocytes apoptosis and oxidation in rats
10.3760/cma.j.issn.0253-3758.2015.03.015
- VernacularTitle:利拉鲁肽可对抗大鼠心肌缺血再灌注损伤
- Author:
Xiangjuan CHENG
1
;
Xiong WANG
;
Xiaohuan TENG
Author Information
1. 山西医科大学第一医院心内科
- Keywords:
Myocardial reperfusion injury;
Liraglutide
- From:
Chinese Journal of Cardiology
2015;43(3):259-263
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of liraglutide on myocardial ischemia/reperfusion (I/R) injury in rats and related mechanisms.Methods Twenty-four male SD rats were divided into sham group,I/R injury group,and liraglutide group by table of random number (n =8 each).Myocardial I/R injury model was established by occlusion of the left anterior descending artery for 30 min followed by 2 h of reperfusion.HE stain method was used to observe cardiomyocyte status under light microscope,myocardial tissue samples were stained with TTC to measure the myocardial infarction size,protein expression of p53 and caspase-3 was analyzed by immunohistochemical technique and Western blot respectively,xanthine oxidase method was used to detect SOD activity,Thiobarbituric acid method was used to measure the concentration of MDA.Results Compared with the I/R group,the degree of myocardial damage of liraglutide group was significantly reduced and the myocardial infarct area was significantly lower ((44 ± 8) ％ vs.(62 ± 8) ％,P ＜ 0.05) while protein expression of caspase-3 and p53 in liraglutide group was significantly downregulated (0.19 ±0.03 vs.0.24 ± 0.02 and 0.27 ± 0.03 vs.0.39 ± 0.04,P ＜ 0.05).SOD activity was significantly increased and MDA concentration was significantly decreased (74.20 ± 11.10 vs.44.04 ± 14.30 and 4.41 ± 1.07 vs.8.72 ± 2.20,P ＜ 0.05) in liraglutide group compared to I/R group.Conclusion Liraglutide protects myocardium against I/R injury possibly through reducing cardiomyocytes apoptosis and oxidation.
