Ad-hVEGF165 reverses homocysteine-induced endothelial dysfunction by regulating nitric oxide system
10.3760/cma.j.issn.0253-3758.2015.05.014
- VernacularTitle:Ad-hVEGF165通过调控一氧化氮系统逆转同型半胱氨酸对内皮细胞的损伤
- Author:
Lili ZHAO
1
;
Yongmin MAO
;
Ying ZHANG
;
Ying SHI
;
Yanqiu SONG
Author Information
1. 300222,天津市心血管病研究所天津市胸科医院
- Keywords:
Vascular endothelial growth factors;
Homocysteine;
Nitric oxide
- From:
Chinese Journal of Cardiology
2015;43(5):432-436
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic effect of Ad-hVEGF165 on the endothelial cells dysfunction induced by homocysteine (Hcy) and related molecular mechanisms.Methods Human umbilical vein endothelial cells CRL-1730 were treated with Hcy at different concentrations (0,0.05,1.00 mmol/L) for 24 h.The same concentration of Hey,Ad-Track and Ad-hVEGF165 were added to the cells in the following groups:blank group,Hcy0.05 group,Hcy1.00 group,Ad-Track group,Hey0.05 + Ad-Track group,Hcy1.00 + Ad-Track group,Ad-hVEGF165 group,Hey0.05 + Ad-hVEGF165 group,Hcy1.00 + AdhVEGF165 group for 48 h.The mRNA and protein expressions of eNOS and DDAH2 were detected by realtime PCR and Western blot.The correlations of mRNA and protein expressions between endothelial nitric oxide synthase (eNOS) and dimethylarginine dimthylaminohydrolase (DDAH)2 were evaluated by Pearson correlation analysis.Results Compared with blank group and Ad-hVEGF465 group,the mRNA and protein expressions of eNOS were decreased in Hcy0.05 group and Hcy0.05 + Ad-hVEGF165 group (both P ＜ 0.05),and the mRNA and protein expressions of DDAH2 in cells treated with 0.05 mmol/L and 1.00 mmol/L Hcy were reduced as well(all P ＜ 0.05).DDAH2 mRNA and protein expression are increased (all P ＜ 0.05) in Ad-hVEGF165 group compared with the blank group and Ad-Track,Hcy0.05 + Ad-hVEGF165 and Hcy0.05 group compared with Hcy0.05 + Ad-Track group,Hcy1.00 + Ad-hVEGF165 and Hcy1.00 group compared with Hcy1.00 + Ad-Track group.The mRNA and protein expressions of eNOS and DDAH2 were uncorrelated under the effect of Hcy(r =0.057 and 0.449,both P ＞0.05) and VEGF (r =0.284 and 0.432,both P ＞ 0.05).Conclusion Recombinant adenovirus Ad-hVEGF165 could reverse Hcy-induced endothelial cells dysfunction via upregulating the expressions of eNOS and DDAH2.
