Impact of mesenchymal stem cells transplantation on myocardial myocardin-related transcription factor-A and bcl-2 expression in rats with experimental myocardial infarction
10.3760/cma.j.issn.0253-3758.2015.06.014
- VernacularTitle:myocardin相关转录因子-A在骨髓间质干细胞治疗心肌梗死中对bcl-2基因的调控作用
- Author:
Ze ZHONG
1
;
Jiaqing HU
;
Yong SUN
;
Jun JIANG
;
Xindong WU
;
Peng XIANG
;
Xiuying LUO
Author Information
1. 311600,浙江大学医学院附属第二医院建德分院心血管内科
- Keywords:
Myocardial infarction;
Stem cell transplantation;
Apoptosis
- From:
Chinese Journal of Cardiology
2015;43(6):531-536
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the impact of mesenchymal stem cells (BMSCs) transplantation on myocardial myocardin-related transcription factor-A (MRTF-A) and bcl-2 expression in rats with experimental myocardial infarction (MI).Methods Thirty rats were randomly divided into sham,MI and MI + BMSCs (1 × 106 injected into 4 infarct points immediately post coronary artery ligation) groups (n =10 each).One week later,TUNEL was used to detect cardiomyocyte apoptosis,the myocardial expression of MRTF-A and bcl-2 was detected by laser scanning confocal microscope and Western blot.In vitro plasmid of MRTF-A and co-transfection with plasmids of MRTF-A and bcl-2 or mutated bcl-2 transfection into cardiomyocyte was applied to evaluate the relationship between MRTF-A and bcl-2.Results The number of apoptotic cardiomyocytes in the sham group,MI group and MI + BMSCs group were (4.05 ± 1.56) ％,(62.38 ± 8.41) ％ and (22.36 ±+ 6.17) ％,respectively (P ＜ 0.05).The protein expression of MRTF-A and bcl-2 in the MI group were significantly lower than those in sham group,while significantly upregulated in MI + BMSCs group (P ＜ 0.05 vs.MI).In cultured neonatal rat cardiomyocyte,the expression of bcl-2 protein was significantly upregulated after transfection with MRTF-A plasmid,and bcl-2-luciferase activity significantly increased after co-transfection with plasmids of MRTF-A and bcl-2-luciferase,however,the positive regulatory effect of MRTF-A was abolished after transfection with mutated bcl-2.Conclusion Mesenchymal stem cells transplantation can effectively reduce cardiomyocyte apoptosis in this rat MI model,and upregulate the expression of MRTF-A.Consequent up-regulated bcl-2 expression might be involved in the beneficial effects of BMSCs transplantation in this model.
