Effects of norepinephrine on proliferation and apoptosis of neonatal cardiac fibroblasts in rats
10.3760/cma.j.issn.0253-3758.2015.06.016
- VernacularTitle:去甲肾上腺素对心肌成纤维细胞增殖及凋亡的影响
- Author:
Miaomiao MA
1
;
Li WANG
;
Yitong MA
;
Yining YANG
;
Bangdang CHEN
;
Xiaoli ZHU
Author Information
1. 832008,新疆石河子大学医学院第一附属医院心内科
- Keywords:
Myocardium;
Fibroblasts;
Norepinephrine;
Cell proliferation;
Apoptosis
- From:
Chinese Journal of Cardiology
2015;43(6):542-547
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of different concentrations of norepinephrine (NE) on proliferation and apoptosis of cultured cardiac fibroblasts (CFBs) from neonatal mice and to elucidate related mechanisms.Methods CFBs of Sprague-Dawley (SD) rats were isolated and cultured and divided into normal control group and different concentration of NE intervention groups (0.1,1,10,50,and 100 μmol/L).Water soluble tetrazolium-1 (WST-1) assay was carried out to detect the viability of CFBs.Morphology of apoptosis cells was evaluated by fluorescence microscope with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining.The expressions of collagen Ⅰ,collagen Ⅲ,prooncogene c-myc in CFBs were detected by reverse transcription-polymerase chain reaction (RT-PCR).The phospho-mitogen activated protein kinase (p-p38MAPK) and caspase3 protein levels were examined by Western blot.Results Proliferation was significantly increased in 1 μmol/L and 10 μmol/L groups compared with the normal control group (1.05 ± 0.05 and 1.09 ± 0.02 vs.1.00 ± 0.03,all P ＜ 0.05).CFBs apoptosis was significantly enhanced in 50 Pμmol/L and 100 μmol/L groups ((22.69 ± 2.18)％ and (36.40 ± 6.80) ％ vs.(4.50 ± 1.08) ％,all P ＜ 0.05).Expression of Collagen Ⅰ peaked in 10 μmol/L group,expression of collagen Ⅲ and c-myc increased dose-dependently in proportion to increasing NE concentrations (all P ＜ 0.05 vs.control group).The expression of p-p38MAPK and caspase3 was also significantly upregulated in a dose-dependent manner in NE groups (all P ＜ 0.05 vs.control group).Conclusions Low concentration NE induces CFBs proliferation and high concentration NE promotes CFBs apoptosis.p38MAPK phosphorylation may be a major mediator of NE-induced effects on CFBs.
