The effect of focal-adhesion micromanipulation in trauma and wound healing.
- Author:
Yu-zhi JIANG
1
;
Jun WEI
;
Bo YUAN
;
Ying-kai LIU
;
Zhi-yong WANG
;
Kui-fu DING
;
Xi-qiao WANG
;
Ming TIAN
;
Chun-sen LI
;
Fei SONG
;
Chun QING
;
Shu-liang LU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Culture Techniques; Cell Growth Processes; Cells, Cultured; Cicatrix; surgery; Dermis; cytology; Female; Fibroblasts; cytology; Focal Adhesions; Humans; Immunohistochemistry; Male; Wound Healing
- From: Chinese Journal of Plastic Surgery 2009;25(1):57-60
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of focal-adhesion micromanipulation on the biological behavior of fibroblast.
METHODSMicro-pot was made by microcontact printing. The molecules of constitutive protein was adhered on micro-pot by self-assemble of peptides. Skin fibroblasts were cultured on the membrane by self-made biomechanical cell culture for 2 weeks. Morphology observation and cell immunohistochemistry analysis was performed.
RESULTSAfter 2 weeks, the morphology of the fibroblasts was diverse and more compliant. Cell immunohistochemistry analysis found that the expression of integrinbeta1, alpha5 and tensin was dramatically reduced.
CONCLUSIONSThe morphology and the biological behaviour of the fibroblasts in hypertrophic scar can be changed after micromanipulation of focal adhesion.
