Effect of botulinum toxin type A injection on hypertrophic scar in rabbit ear model.
- Author:
Lin WANG
1
;
Ning-Zheng TAI
;
Zhi-Hong FAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Botulinum Toxins, Type A; pharmacology; Cells, Cultured; Cicatrix, Hypertrophic; metabolism; pathology; Ear; pathology; Female; Fibroblasts; drug effects; Injections, Subcutaneous; Rabbits; Wound Healing; drug effects
- From: Chinese Journal of Plastic Surgery 2009;25(4):284-287
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of botulinum toxin type A (Botox A) injection on hypertrophic scar in rabbit ear model.
METHODSThe hypertrophic scar model was established in 16 Japanese rabbits' ears. These wounds were divided into two groups as group T (treated with Botox A, n = 48) and group S (not treated, n = 48). The wounds healing times and scar hypertrophy were observed with 8 specimen of normal skin at the rabbit ears as sham group B. HE stain was used to assess the hypertrophic index (HI). The expression of collagen I and III was tested by western-blot. The cell cycle of fibroblasts was studied by flow cytometry.
RESULTSThe HI was significantly lower in group T than in group S (P < 0.01). The expression of collagen I and III, as well as the ratio of I to III, was markedly stronger in group S than in group T (P < 0.01). Compared with group T, more fibroblasts were in G2-M in group S and fewer in G0-G1 (P < 0.05).
CONCLUSIONSLocal injection of Botox A can inhibit the formation of hypertrophic scar and the activity of fibroblasts in rabbit ear model. It can significantly decrease the expression of collagen I and III in hypertrophic scar, as well as the ratio of collagen I to III. It serves as the basis for the treatment of hypertrophic scar with Botox A.
