Proliferation of the mesenchymal stem cells in a delayed fat flap: an experimental study in rabbits.
- Author:
Su-Jun JIANG
1
;
Xiao-Sheng HE
;
You-Di NI
;
Mao-Lin LIU
;
Su-Hang LIU
;
Xiao-Chun ZHONG
Author Information
- Publication Type:Journal Article
- MeSH: Adipose Tissue; cytology; transplantation; Animals; Cell Proliferation; Cells, Cultured; Graft Survival; Postoperative Period; Rabbits; Stem Cells; cytology; Surgical Flaps
- From: Chinese Journal of Plastic Surgery 2009;25(4):287-289
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the possibility to enhance the proliferation of adipose-derived stem cells (ASCs) in a delayed fat flap in rabbits.
METHODSA delayed fat flap was formed in one side of inguinal region of a rabbit. 21 days after operation, the fat tissues at the delayed flaps and at the unoperated side were harvested and digested with 0.25% collagenase and sieved. The cell suspensions were centrifuged. The cells were obtained from tissue precipitate after centrifugation. The expression rates of the surface marker (CD29, CD44, CD14 and CD45) were measured by FCM and compared between the experimental and control groups.
RESULTSExpression rates of CD29 and CD44 were higher in the delayed fat flap (74.06% and 90.74%) than in the contralateral fat tissue (62.88% and 77.54%, P < 0.05), while those of CD14 and CD45 were lower in the delayed fat flap (57.66% and 4.84%) than in the contralateral fat tissue (72.10% and 75.82%, P < 0.05 and P < 0.01).
CONCLUSIONSTissue hypoxic ischemia such as fat tissue in a delayed fat flap can promote proliferation of ASCs. It indicates that tissue in the delayed flap may be transplanted with better survival rate. The ischemia pretreatment of fat tissue may become a new method for fat transplantation.
