Migration of intravenously injected adipose tissue-derived stem cells in SD rats with soft tissue wound.
- Author:
Hua NAN
1
;
Jian-Hua GAO
;
Feng LU
;
Yun-Jun LIAO
Author Information
- Publication Type:Journal Article
- MeSH: Adipocytes; cytology; Animals; Cell Movement; Male; Rats; Rats, Sprague-Dawley; Stem Cell Transplantation; Stem Cells; cytology; Wound Healing; Wounds and Injuries
- From: Chinese Journal of Plastic Surgery 2009;25(5):372-376
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the migration of intravenously injected adipose tissue-derived stem cells (ADSCs) in SD rats with soft tissue wound.
METHODS1.8 cm x 1.8 cm full thickness skin defect and 0.5 cm in depth soft tissue defects were made on the back of 6 SD rats. ADSCs were isolated and cultured in vitro for 3 passages. 2.4 x 10(6) ADSCs were labeled with DiI and transplanted into the SD rats through tail vein. Normal skin and wound tissue samples were collected for fluorescent distribution observation 24, 48 days after injection, respectively.
RESULTSCompared to normal skin, more fluorescent positive cells were detected in the margin and deep layer of the wound 24 days after operation. But it is accumulated within dermis and adenoid 48 days after operation.
CONCLUSIONWound can probably induce the migration and accumulation of intravenously transplanted ADSCs.
