Influence of AML1 and AML1-ETO on transcriptional regulation of nucb2 gene.
- Author:
Bei ZOU
1
;
Wen JIN
;
Jing LI
;
Jian-Tao SHI
;
Ji ZHANG
;
Kan-Kan WANG
Author Information
1. National Key Laboratory of Medical Genomics, Ruijin Hospital, Shanghai Jiaotong University Medical College, Shanghai 200025, China.
- Publication Type:Journal Article
- MeSH:
Calcium-Binding Proteins;
genetics;
Cell Line, Tumor;
Core Binding Factor Alpha 2 Subunit;
genetics;
DNA-Binding Proteins;
genetics;
Gene Expression Regulation, Leukemic;
Humans;
Nerve Tissue Proteins;
Oncogene Proteins, Fusion;
genetics;
Promoter Regions, Genetic;
RUNX1 Translocation Partner 1 Protein;
Transcription Factors;
genetics;
Transcriptional Activation
- From:
Journal of Experimental Hematology
2009;17(6):1482-1486
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to investigate the effect of AML1-ETO fusion protein resulted from hematopoietic transcription factor (AML1) and acute myeloid leukemia M(2b)(AML-M(2b)) on transcription activity of nucleobindin 2 (nucb2) promoter, and to explore the role of AML1-ETO in molecular pathogenesis of AML-M(2b). The real-time RT-PCR was used to study the regulation of AML1-ETO on nucb2 at transcription level in AML1-ETO inducible leukemia cell line, the chromatin immunoprecipitation (ChIP)-based qPCR was used to investigate the direct in vivo interaction between the AML1, AML1-ETO and nucb2 promoter in AML1-ETO positive leukemia cell line, the luciferase report gene assay was used to detect the regulation of AML1, AML1-ETO on the transcription activity of nucb2 promoter. The results showed that the expression level of nucb2 was reduced with the increase of AML1-ETO. The promoter of nucb2 could be bound by both AML1 and AML1-ETO. The promoter of nucb2 was trans-repressed by AML1 and AML1-ETO respectively. It is concluded that the nucb2 is the direct target gene of AML1 and AML1-ETO, the transcription regulation of AML1, AML1-ETO on nucb2 is carried out via repressing its promoter activity.
