Effect of ATRA on the expression of genes Hoxb2 and Hoxb4 in cord blood erythroid progenitors.
- Author:
Cui-Qiong DU
1
;
Mei-Xian HUANG
;
Wen-Jun LIU
Author Information
1. Department of Pediatrics, Quanzhou Medical College, Quanzhou 362000, Fujian Province, China.
- Publication Type:Journal Article
- MeSH:
Cells, Cultured;
Female;
Fetal Blood;
cytology;
Gene Expression;
drug effects;
Hematopoietic Stem Cells;
drug effects;
Homeodomain Proteins;
genetics;
Humans;
Pregnancy;
Transcription Factors;
genetics;
Tretinoin;
pharmacology
- From:
Journal of Experimental Hematology
2009;17(6):1516-1521
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the expressions of genes hoxb2 and hoxb4 after interference of the proliferation and differentiation of hematopoietic stem cells (HSC) to the erythroid progenitors (CFU-E) in vitro by using all-trans retinoic acid (ATRA). The cord blood was collected from 12 cases of fetal placenta umbilical vein and cultured by using culture technique of HSC in vitro. The proliferation and differentiation of HSC to CFU-E were interfered with 6 x 10(-8) mol/L of ATRA. The expression levels of genes hoxb2 and hoxb4 in blank control and ATRA groups were detected by FQ-RT-PCR on day 3, 7 and 10 of culture. The results showed that the expressions of genes Hoxb2 and hoxb4 were a little on day 3, obviously increased on day 7 and reached highest level on day 10 in 2 groups. The expression level of hoxb4 on day 3, 7 and 10 in blank control group was obviously higher than expression level of hoxb2. As compared with blank control group, the expressions of genes hoxb2 and hoxb4 in the ATRA group were significantly up-regulated. It is concluded that the genes hoxb2 and hoxb4 all expressed in process of proliferation and differentiation to erythroid progenitors, which suggests that hoxb2 and hoxb4 relate to erythroid hematopoiesis, and the hoxb4 has more great relevance to erythroid hematopoiesis as compared with hoxb2. The ATRA (6 x 10(-8) mol/L) can up-regulate the expression of hoxb2 and hoxb4 significantly.
