Effect of recombinant human tumor necrosis factor alpha on activity of gelatinase B and migration of human umbilical cord blood mononuclear cells in vitro.
- Author:
Ting WANG
1
;
Xin LIU
;
Rui HU
Author Information
1. Department of Hematology, The First Hospital, Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Cell Movement;
drug effects;
Cells, Cultured;
Female;
Fetal Blood;
cytology;
Humans;
Matrix Metalloproteinase 9;
metabolism;
Pregnancy;
Recombinant Proteins;
pharmacology;
Tumor Necrosis Factor-alpha;
administration & dosage;
pharmacology
- From:
Journal of Experimental Hematology
2009;17(6):1532-1537
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the activity of matrix metalloproteinase-9 (MMP-9) in umbilical cord blood mononuclear cells, the effect of recombinant human tumor necrosis factor alpha (rhTNF-alpha) on the activity of MMP-9 and cell migration capability of umbilical cord blood mononuclear cells, as well as the relationship between them. The different concentrations of TNF-alpha was added into the mononuclear cells. The activity of MMP-9 in umbilical cord blood mononuclear cells were analyzed by gelatinase spectrum. The cell migration capability was observed through the millicell carbine. The results showed that after umbilical cord blood mononuclear cells were cultured in serum-free RPMI 1640 medium for 24 and 48 hours, the activity of MMP-9 could be detected, the cell migration capability at 24 and 48 hours were (1.371 +/- 0.011)% and (1.384 +/- 0.014)% respectively, there was no statistical difference between them. Large dose of TNF-alpha (500 U/ml) led to the death of the cells; small doses of TNF-alpha (< 200 U/ml) up-regulated the activity of MMP-9, and as the concentration of TNF-alpha increased, the activity of MMP-9 was enhanced. Small doses of TNF-alpha (< 200 U/ml) improved the migration capability of umbilical cord blood mononuclear cells in a dose-dependent manner. It is concluded that small doses of TNF-alpha can upregulate the activity, enhance the migration capability of umbilical cord blood mononuclear cells, which probably accelerates cell homing after umbilical cord blood stem cell transplant and improves the hematopoietic reconstruction after transplantation.
