Factors impacting yield of CD34(+) cells from healthy donors mobilized with rhG-CSF.
- Author:
Ling ZHU
1
;
Li-Kun ZHOU
;
Mei XUE
;
Hong-Min YAN
;
Jing LIU
;
Zhi-Dong WANG
;
Li DING
;
Heng-Xiang WANG
Author Information
1. Department of Hematology, Air Force General Hospital, Chinese PLA, Beijing 100036, China. zhuling2006@gmail.com
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Age Factors;
Antigens, CD34;
immunology;
metabolism;
Blood Cell Count;
Blood Cells;
cytology;
immunology;
Body Height;
Body Mass Index;
Body Weight;
Child;
Female;
Granulocyte Colony-Stimulating Factor;
administration & dosage;
Hematopoietic Stem Cell Mobilization;
methods;
Hematopoietic Stem Cell Transplantation;
Humans;
Male;
Middle Aged;
Recombinant Proteins;
Sex Factors;
Tissue Donors;
Young Adult
- From:
Journal of Experimental Hematology
2009;17(6):1541-1545
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to explore the effect of donor characteristics (age, sex and so on.) on CD34(+) cell yields in apheresis from healthy donors mobilized by recombinant granulocyte colony-stimulating factor(rhG-CSF). In 61 healthy donors, the characteristics associated with CD34(+) cell yield were analysed. The relationship between the CD34(+) cell yields and donor characteristics was statistically assessed with multivariate forward, backward and stepwise regression methods. A variety of parameters were analyzed which included donor age, sex, weight, height, body mass index (BMI) and time for collection of peripheral blood apheresis, while the mean number of peripheral blood mononuclear cells (MNCs), CD34(+) cell count, CD34(+) cell proportion based on MNC and CD34(+) cell count per kg of donor weight were used as the variables. The results showed that age of donors was the main factor impacting CD34(+) cell yields (-0.60 < r < -0.45, p < 0.005). In a partial correlation analysis the body weight, height and BMI were served as control factors, the negative correlation of age with CD34(+) cell yields was still found (-0.50 < r < -0.35, p < 0.02). BMI was only weakly correlated with the yields of CD34(+) cells per kg(r = -0.297, p < 0.05). As a whole, sex showed no relation with the CD34(+) cell yields. Compared with the female group less than 35 years old, height, weight and BMI in male group of low age exerted a positive impact on CD34(+) cell yield. The optimal time for collection of PB was day 4 after treatment with rhG-CSF, when 70% of the donors could reach the peak CD34(+) cell yields. It is concluded that the age of the donors is the first factor determining the choice of donors for allogeneic hematopoietic stem cell transplantation, the sex, height, weight and BMI are secondary factors impacting yield of CD34(+) cells from donors mobilized with rhG-CSF.
