Effect of inflammatory cytokines on the expression of Krupple-like factor 4 in endothelium.
- Author:
Rui-Juan ZHANG
1
;
Lin-Hua YANG
Author Information
1. Department of Hematology, The Second Hospital, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China.
- Publication Type:Journal Article
- MeSH:
Cells, Cultured;
Endothelium;
metabolism;
Humans;
Interleukin-1beta;
pharmacology;
Kruppel-Like Transcription Factors;
metabolism;
RNA, Messenger;
metabolism;
Tumor Necrosis Factor-alpha;
pharmacology
- From:
Journal of Experimental Hematology
2009;17(6):1551-1554
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to investigate the expression of Krupple-like factor 4 (KLF-4) in human umbilical vein endothelial cells (HUVEC) under stimulating of cytokines IL-1beta and TNF-alpha. The HUVEC were isolated, cultured and exposed to IL-1beta, TNF-alpha for 2 and 4 hours, the expression level of KLF-4 mRNA was detected by real-time PCR, the expression level of KLF-4 protein was measured by Western blot, the location of KLF-4 in HUVEC was determined by confocal laser microscopy. The results showed that the KLF-4 mRNA lowly expressed in normal HUVEC, but highly expressed in HUVEC after stimulation with IL-1beta and TNF-alpha; furthermore the expressions of KLF-4 mRNA and protein were enhanced along with prolonging of time. Confocal laser microscopy showed that the expression of KLF-4 was enhanced in nucleus and plasma of HUVEC after stimulating with IL-1beta. It is concluded that the expression of KLF-4 increases under IL-1beta and TNF-alpha stimulation which may participate in regulation of endothelial activity.
