Establishment of Chinese hamster ovary cell line expressing recombinant GPIb-IX complex.
- Author:
Yi LIAO
1
;
Wei-Lin ZHANG
;
Yan-Hong YUAN
;
Quan-Wei SHI
;
Su-Ping LI
;
Rong YAN
;
Zhi-Cheng WANG
;
Ke-Sheng DAI
Author Information
1. School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
- Publication Type:Journal Article
- MeSH:
Animals;
CHO Cells;
Cricetinae;
Cricetulus;
Mutation;
Plasmids;
Platelet Glycoprotein GPIb-IX Complex;
biosynthesis;
genetics;
Recombinant Proteins;
biosynthesis;
genetics
- From:
Journal of Experimental Hematology
2009;17(6):1560-1563
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to construct Chinese Hamster Ovary (CHO) cell models expressing recombinant wild-type GPIb-IX and mutant GPIb-IX complex, so as to provide the platform to study the related physiologic functions of GPIb-IX. The plasmids were extracted from E.coli expressing wild-type or deletion mutant GPIbalpha and were identified by digestion with EcoR I. Three plasmids containing GPIbalpha, GPIbbeta, and GPIX genes were co-transfected into CHO cells, and then the expression of GPIb-IX complex was detected by immune coprecipitation, Western blot and flow cytometry. The results showed that the expression of GPIb-IX complex could be detected in the lysate and on the surface of CHO cells at 48 hours after transfection. In conclusion, CHO cell models expressing recombinant wild-type or mutation GPIb-IX complex has been successfully constructed.
