Correlation between mTOR signaling transduction pathway and arsenic trioxide response.
- Author:
Fei JIANG
1
;
Zhuo-Gang LIU
Author Information
1. Department of Hematology, Shengjing Hospital, China Medical Univercity, Shenyang 110004, Liaoning Province, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Arsenicals;
pharmacology;
Humans;
K562 Cells;
Oxides;
pharmacology;
Signal Transduction;
TOR Serine-Threonine Kinases;
metabolism
- From:
Journal of Experimental Hematology
2010;18(1):54-56
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the correlation between mTOR signaling transduction pathway and arsenic trioxide (ATO) effect. The expressions of pmTOR, pAKT and pP70S6K in K562/DNR treated with ATO for different time were detected by Western blot. The apoptosis rate of K562/DNR treated by ATO combined with LY294002 or rapamycin for 120 hours was assayed by flow cytometry. The results showed that the expression of pmTOR in K562/DNR cells treated with ATO for 60 minutes or 120 minutes was higher than that in the control group (p < 0.01); the expressions of pAKT in the cells treated with ATO for 30 minutes or 60 minutes were higher than that in the control group (p < 0.01); the expression of pP70S6K in the cells treated with ATO for 60 minutes was higher than that in the control group (p < 0.01). The apoptosis rate of K562/DNR cells treated with combination of ATO and LY294002 or rapamycin were higher than that in the control group. It is concluded that the mTOR signaling pathway in K562/DNR cells is activated by a certain concentration of ATO, and mTOR signaling pathway inhibitors enhance ATO to trigger apoptosis in K562/DNR cells.
