Induction of apoptosis of leukemic tumor cells in mouse model with G-quadruplex ligand Tel03.
- Author:
Bin CHU
1
;
Ying ZHANG
;
Hong-Xing LIU
;
Yan-Xia BAI
;
Xing-Guo ZUO
;
Min-Qiu LU
;
Meng-Qing WU
;
Lei SHI
;
Lei LIU
;
Ping ZHU
Author Information
1. Department of Hematology, Beijing Jishuitan Hospital, Beijing 100035, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
Female;
G-Quadruplexes;
Humans;
K562 Cells;
Mice;
Mice, Inbred BALB C;
Mice, Nude;
Proto-Oncogene Proteins;
metabolism;
Proto-Oncogene Proteins c-bcl-2;
Xenograft Model Antitumor Assays;
bcl-2-Associated X Protein;
metabolism
- From:
Journal of Experimental Hematology
2010;18(1):57-60
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the anti-leukemia activity of Tel03 in vivo. The K562 xenografted leukemia model was established and mice were divided randomly into three groups. Mice of different group were treated with PBS (control), 5 mg/kg Tel03 or 15 mg/kg Tel03 (ip, twice a week) respectively. Tumor volume, body weight and other behavior were observed regularly. Cell apoptosis was detected with TUNEL assay and the expression levels of Bcl-2 and Bax were detected by Western blot. The results indicated that Tel03 exerted anti-leukemia activity in mouse model. Tel03 significantly reduced tumor volume in Tel03-treated group compared with control. In addition, 5 mg/kg Tel03 induced cell apoptosis without exerting apparent toxicity in mice. After Tel03 treatment, the expression of Bcl-2 was inhibited, however, the expression of Bax was up-regulated. It is concluded that G-quadruplex ligand Tel03 can induce cell apoptosis in leukemia mouse model, and this agent may be a potential anticancer drug.
