Changes of HLA-DR15 and immunoglobulin, T lymphocyte subsets in patients with aplastic anemia, myelodysplastic syndrome and their significance.
- Author:
Jiang-Xia CUI
1
;
Min-Fei PEI
;
Guang-Sen ZHANG
;
Min XU
Author Information
1. Department of Hematology, The Sencond Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Aged, 80 and over;
Anemia, Aplastic;
immunology;
metabolism;
Case-Control Studies;
Female;
Flow Cytometry;
HLA-DR Antigens;
immunology;
metabolism;
HLA-DR Serological Subtypes;
Histocompatibility Testing;
Humans;
Immunoglobulins;
immunology;
metabolism;
Male;
Middle Aged;
Myelodysplastic Syndromes;
immunology;
metabolism;
T-Lymphocyte Subsets;
immunology;
metabolism;
Young Adult
- From:
Journal of Experimental Hematology
2010;18(1):111-115
- CountryChina
- Language:Chinese
-
Abstract:
The objective of this study was to detect the expression frequency of HLA-DR15 in patients with aplastic anemia (AA) and myelodysplastic syndrome (MDS), to investigate the relation of expression frequency with diseases and to analyze the relationship between immunoglobulin, T lymphocyte subsets and HLA-DR15. HLA-DR15 expression was detected by PCR-SSP; immunoglobulin was detected by immune turbidimetry; T cell subsets were detected by flow cytometry. The results showed that the expression rates of HLA-DR15 in AA and MDS as well as normal control groups were 78.6%, 63.2% and 24.6% respectively. The difference between AA, MDS and the normal control groups was statistically significant (p < 0.01). OR (odds ratios) values of AA and MDS groups were 11.262, 4.710 respectively. Compared with normal control group, expression rate of HLA-DR15 in hematologic malignancy group was not significantly different. The immunoglobulin level and abnormal T cell subsets in AA and MDS groups were statistically different between HLA-DR15 positive and negative groups (p > 0.05). It is concluded that the frequency of HLA-DR15 antigen in AA and MDS patients is significantly higher than that in normal control and hematologic malignancy group. OR value>1 showed a positive correlation between the diseases and HLA-DR15. HLA-DR15 is a susceptible gene in AA and MDS. The abnormalities of immunoglobulin level and ratios of T cell subsets in AA and MDS are common, but are not associated significantly with the expression of HLA-DR15.
