The effects of wild-type PTEN transfection on gene expressions of glioblastomas.
- Author:
Xinxia TIAN
1
;
Jinfen WANG
;
Jinxia ZHANG
;
Juan DU
;
Chung Sean PANG
;
N G HO-KEUNG
Author Information
- Publication Type:Journal Article
- MeSH: Brain Neoplasms; genetics; Gene Expression; Gene Expression Regulation, Neoplastic; Glioblastoma; genetics; Humans; Oligonucleotide Array Sequence Analysis; PTEN Phosphohydrolase; Phosphoric Monoester Hydrolases; genetics; physiology; Transfection; Tumor Cells, Cultured; Tumor Suppressor Proteins; genetics; physiology
- From: Chinese Journal of Pathology 2002;31(1):46-49
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of wild-type PTEN on gene expressions of glioblastomas.
METHODSGlioblastoma U87MG cells, which express inactivated PTEN, were transfected with wild-type PTEN constructs and stable transfected clones were selected. Then, cDNA microarray analyses were used to identify differentially expressed genes in wild-type PTEN transfected cells and control cells.
RESULTSTransfected wild-type PTEN inhibited the proliferation of U87MG. By cDNA microarray analyses, 89 cDNA clones were identified, which were differentially expressed in wild-type PTEN transfected cells and control cells. Among these genes, 13 genes were unknown and 76 genes were known genes, including glial fibrillary acidic protein, p21/WAF1, human TGF-beta inducible early protein, human DNA fragmentation factor 45 etc.
CONCLUSIONWild-type PTEN can affect the expressions of multiple genes, by which it regulates the proliferation, differentiation and apoptosis of glioblastomas.
