Effects of andrographolide on the activation of mitogen activated protein kinases and nuclear factor-κB in mouse peritoneal macrophage-derived foam cells.
- Author:
Fu-xing LI
1
;
Shu-sheng LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Anti-Inflammatory Agents; pharmacology; Atherosclerosis; immunology; metabolism; prevention & control; Cells, Cultured; Diterpenes; pharmacology; Extracellular Signal-Regulated MAP Kinases; metabolism; Foam Cells; cytology; drug effects; enzymology; JNK Mitogen-Activated Protein Kinases; metabolism; Lipoproteins, LDL; metabolism; MAP Kinase Signaling System; drug effects; immunology; Macrophages, Peritoneal; cytology; drug effects; enzymology; Mice; Mice, Inbred Strains; NF-kappa B; metabolism; Vasculitis; drug therapy; immunology; metabolism; p38 Mitogen-Activated Protein Kinases; metabolism
- From: Chinese journal of integrative medicine 2012;18(5):391-394
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo observe the effect of andrographolide on the activation of mitogen-activated protein kinases (MAPKs) and expression of nuclear factor-κB (NF-κB) in macrophage foam cells.
METHODSThe mouse peritoneal macrophages were cultured in the media in the presence of oxidized low-density lipoprotein (ox-LDL), ox-LDL+andrographolide, or neither (control). The phosphorylation of MAPK molecules (p38MAPK, JNK, ERK1/2) and the expressions of NK-κB p65 were examined by Western blot.
RESULTSAs compared with cells in the control group, the expressions of phospho-p38 and NF-κB p65 were increased in the cells cultured with either ox-LDL or ox-LDL+andrographolide (P<0.01), but attenuated significantly in the presence of ox-LDL+ andrographolide when compared with ox-LDL (P<0.05). The phospho-JNK increased in the presence of either ox-LDL or ox-LDL+andrographolide when compared with control cells (P<0.01), but no significant difference existed between ox-LDL and ox-LDL+andrographolide (P>0.05). The expression of phospho-ERK1/2 was increased in the presence of ox-LDL compared with the control cells (P<0.01), but no significant differences existed between the cells cultured in the presence of ox-LDL+andrographolide and the control medium (P>0.05).
CONCLUSIONSAndrographolide could inhibit the activation of ERK1/2, p38MAPK and NK-κB induced by ox-LDL in macrophage foam cells, which might be one of its mechanisms in preventing atherosclerosis.
