Realgar-induced apoptosis of cervical cancer cell line Siha via cytochrome c release and caspase-3 and caspase-9 activation.
- Author:
Yan-xiang CHENG
1
;
Rong LIU
;
Qin WANG
;
Bing-shu LI
;
Xue-xian XU
;
Min HU
;
Lu CHEN
;
Qiong FU
;
De-min PU
;
Li HONG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; physiology; Arsenicals; pharmacology; Caspase 3; metabolism; Caspase 8; metabolism; Caspase 9; metabolism; Caspase Inhibitors; Cell Line, Tumor; Cell Survival; drug effects; physiology; Cytochromes c; metabolism; Dose-Response Relationship, Drug; Enzyme Activation; drug effects; Enzyme Inhibitors; pharmacology; Female; Fluorescence Resonance Energy Transfer; Humans; Sulfides; pharmacology; Uterine Cervical Neoplasms; drug therapy; metabolism; pathology
- From: Chinese journal of integrative medicine 2012;18(5):359-365
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo explore the molecular mechanism of realgar-induced apoptosis of cervical cancer cells.
METHODSThe cervical cancer cell line Siha was used to determine the cell viability and apoptosis after treatment with realgar using MTT assay and flow cytometry. The activities of caspase-3, -8, and -9 were detected by fluorescence resonance energy transfer technology and colorimetric assay, while the levels of Bcl-2, cytochrome c, and Bax were detected by Western blot method.
RESULTSInduction of apoptosis by realgar was detected in Siha cell line in a dose-dependent manner. The apoptosis was accompanied by a significant increase in cytochrome c release and activation of caspase-3 and caspase-9 but not caspase-8. Further, the realgar-induced apoptosis was inhibited by a broad-spectrum caspase inhibitor, a caspase-3 inhibitor, and a caspase-9 inhibitor but not by a caspase-8 inhibitor. Bcl-2 and Bax protein expressions were not changed by realgar.
CONCLUSIONThe induction of apoptosis by realgar is mediated through a cytochrome c-dependent pathway, which sequentially activates caspase-9 and caspase-3.
