Correlation between Fc γ R III a and aortic atherosclerotic plaque destabilization in ApoE knockout mice and intervention effects of effective components of chuanxiong rhizome and red peony root.
- Author:
Ye HUANG
1
;
Hui-jun YIN
;
Xiao-juan MA
;
Jing-shang WANG
;
Qian LIU
;
Cai-feng WU
;
Ke-ji CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aorta; drug effects; enzymology; pathology; Apolipoproteins E; deficiency; metabolism; Drugs, Chinese Herbal; pharmacology; therapeutic use; Flow Cytometry; Gene Expression Regulation, Enzymologic; drug effects; Lipopolysaccharide Receptors; metabolism; Male; Matrix Metalloproteinase 9; genetics; metabolism; Mice; Mice, Inbred C57BL; Mice, Knockout; Monocytes; drug effects; metabolism; Paeonia; chemistry; Phytotherapy; Plant Roots; chemistry; Plaque, Atherosclerotic; blood; drug therapy; enzymology; pathology; RNA, Messenger; genetics; metabolism; Receptors, IgG; metabolism; Tumor Necrosis Factor-alpha; blood
- From: Chinese journal of integrative medicine 2011;17(5):355-360
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo explore the correlation between Fc γ RIII A (CD16A) and aortic atherosclerotic plaque destabilization in apoE knockout (apoE KO) mice and the intervention effects of effective components of chuanxiong rhizome and red peony root.
METHODSEight 8-week-old male C57BL/6J mice were selected as the control group. Forty 8-week-old male apoE KO mice were randomly divided into the model group, apoE KO + intraperitoneal injection immunoglobulin group (IVIG), apoE KO + simvastatin group (Sm), apoE KO + high dosage of xiongshao capsule (XSC) group (XSCH), and apoE KO + low dosage of XSC group (XSCL), 8 mice in each group. Mice in the control group were put on a normal diet, and others were fed with a high-fat diet. After 10-week different interventions, monocyte CD16 expression was detected by flow cytometry, aortic matrix metalloproteinase-9 (MMP-9) mRNA expression was detected using reverse transcription polymerase chain reaction, and serum tumor necrosis factor (TNF)-α level was detected using enzyme-linked immunosorbent assay.
RESULTSCompared with the control group, monocyte CD16 expression, aortic MMP-9 mRNA expression, and serum TNF-α level in the model group increased obviously (P<0.01). Injections of apoE KO mice with intraperitoneal immunoglobulin during a 5-day period significantly reduced the monocyte CD16 expression, aortic MMP-9 mRNA expression, and serum TNF-α level (P<0.01 or 0.05) over a 10-week period of high-fat diet. Indices above in the Sm group, XSCH group, and XSCL group decreased in a different degree. Of them, the aortic MMP-9 mRNA expression in XSCH group was lower than that in Sm group (P<0.05) and the monocyte CD16 expression and serum TNF-α level showed no significant difference between XSCH group and Sm group (P>0.05). Correlation analyses suggested positive correlation between monocyte CD16 expression and aortic MMP-9 mRNA expression or serum TNF-α level in IVIG group, XSCH group, and XSCL group.
CONCLUSIONSFcγR III A mediates systemic inflammation in the progression of coronary heart disease with blood stasis syndrome. XSC could stabilize atherosclerotic plaque by suppressing inflammation and its target was relative with FcγRIII A.
