Pien Tze Huang induced apoptosis in human colon cancer HT-29 cells is associated with regulation of the Bcl-2 family and activation of caspase 3.
- Author:
Jiu-mao LIN
1
;
Li-hui WEI
;
You-qin CHEN
;
Xian-xiang LIU
;
Zhen-feng HONG
;
Thomas J SFERRA
;
Jun PENG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Caspase 3; metabolism; Cell Proliferation; drug effects; Cell Survival; drug effects; Colonic Neoplasms; enzymology; pathology; Drug Screening Assays, Antitumor; Drugs, Chinese Herbal; pharmacology; Enzyme Activation; drug effects; HT29 Cells; Humans; Membrane Potential, Mitochondrial; drug effects; Proto-Oncogene Proteins c-bcl-2; metabolism; bcl-2-Associated X Protein; metabolism
- From: Chinese journal of integrative medicine 2011;17(9):685-690
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the cellular effects of Pien Tze Huang (PZH) in the HT-29 human colon carcinoma cell line.
METHODSThe viability of HT-29 cells was determined by MTT assay. A fluorescence-activated cell sorting (FACS) analysis with annexin-V/propidium iodide (PI) and JC-1 staining were performed to determine cell apoptosis and the loss of mitochondrial membrane potential, respectively. Activation of caspase 3 was evaluated by a colorimetric assay. The mRNA expression levels of Bcl-2 and Bax were measured by reverse transcription polymerase chain reaction (RT-PCR).
RESULTSPZH, in a dose- and time-dependent manner, reduced viability and induced apoptosis of HT-29 cells. Moreover, PZH treatment resulted in the collapse of the mitochondrial membrane potential, activation of caspase 3, and an increase in the Bax/Bcl-2 ratio.
CONCLUSIONPZH inhibits the growth of HT-29 cells by inducing cancer cell apoptosis via regulation of the Bcl-2 family and activation of caspase 3, which may, in part, explain its anticancer activity.
