Fuzheng Yiliu Granule inhibits the growth of hepatocellular cancer by regulating immune function and inducing apoptosis in vivo and in vitro.
- Author:
Zhi-yun CAO
1
;
Xu-zheng CHEN
;
Lian-ming LIAO
;
Jun PENG
;
Hai-xia HU
;
Zhi-zhen LIU
;
Jian DU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Carcinoma, Hepatocellular; blood; immunology; pathology; Cell Proliferation; drug effects; Cell Survival; drug effects; Drugs, Chinese Herbal; pharmacology; Hep G2 Cells; Humans; Interleukin-2; biosynthesis; Liver Neoplasms; blood; immunology; pathology; Lymphocyte Count; Male; Mice; Mice, Inbred ICR; Rats; Serum; Tumor Burden; drug effects; Tumor Necrosis Factor-alpha; biosynthesis; Xenograft Model Antitumor Assays
- From: Chinese journal of integrative medicine 2011;17(9):691-697
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the inhibitory effect of Fuzheng Yiliu Granule (FYG) on hepatocellular cancer (HCC) and investigate the mechanism mediating its bioactivity.
METHODSH22 tumor-bearing ICR mice were treated with FYG [3.6 g/(kg·d)] for 5 days. Tumor volume and tumor weight, percentages of CD3(+), CD4(+), CD8(+), and natural killer (NK) cells in peripheral blood, tumor apoptosis and serum levels of interleukin-2 (IL-2), and tumor necrosis factor-α (TNF-α) were evaluated. FYG-containing serum was prepared from SD rats treated for 7 days [high dose 3.6 g/(kg·d); middle dose 1.8 g/(kg·d); low dose 0.9 g/(kg·d)]. Cell cycle, cell viability, and apoptosis were evaluated after HepG2 cell line was cultured in FYG-containing serum for 48 h. The levels of IL-2 and TNF-α in FYG-containing serum were also determined.
RESULTSFYG produced a potent antitumor effect (P<0.01) and induced marked apoptosis of the tumor tissue (P<0.05). Mice treated with FYG had higher percentages of CD3(+) and CD4(+) (P<0.05), and more NK cells (P<0.01) in the peripheral blood than those in the animals treated with normal saline. Mice receiving FYG had the highest serum levels of IL-2 and TNF-α (P<0.01). High-dose FYG-containing serum significantly decreased HepG2 cell viability, inhibited cell proliferation (P<0.05), and induced apoptosis (P<0.01). In addition, the levels of IL-2 and TNF-α of high-dose-containing serum were higher than the blank serum (P<0.01).
CONCLUSIONFYG could inhibit HCC growth by regulating immune function and inducing apoptosis of tumor cells in vivo and in vitro.