Effects of Nanofiber Alignment and Strain Direction on Cellular Activities of Human ACL Fibroblasts.
- Author:
Chang Hun LEE
1
;
Ho Joon SHIN
;
In Hee CHO
;
Young Mi KANG
;
In Ae KIM
;
Jung Woog SHIN
Author Information
1. Department of Biomedical Engineering, Inje University, Gimhae, Gyeongnam, Korea. sjw@bme.inje.ac.kr
- Publication Type:Original Article
- Keywords:
Ligament tissue engineering;
Aligned nanofiber;
Biomimetic scaffold;
Mechanical stimuli;
Strain direction
- MeSH:
Biomimetics;
Collagen;
Fibroblasts*;
Humans*;
Ligaments;
Nanofibers*
- From:Journal of Korean Orthopaedic Research Society
2004;7(1):1-12
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The effects of fiber alignment and direction of mechanical strain on the ECM generation of human ACL fibroblast were assessed. MATERIALS AND METHODS: The aligned nanofiber was fabricated using electrospinning with a rotating target. The amounts of collagen on aligned and randomly oriented structures were compared. To evaluate the effect of strain direction, 5% uniaxial strain (0.2 Hz) was applied to fibroblasts seeded on parallel aligned, vertically aligned to the strain direction, and randomly oriented nanofiber sheets. The amounts of collagen produced were measured 2 days after halting the strain application. RESULTS: The fibroblasts on the aligned nanofiber were spindle-shaped and oriented in the direction of the fibers. Significantly more collagen (22.5+/-2.7 ug/ngDNA) was synthesized on the aligned nanofiber than the randomly oriented (14.5+/-3.2 ug/ngDNA). And the amounts of collagen produced were increased by 150% and 50% approximately with the longitudinal and perpendicular cyclic strain, respectively. CONCLUSION: The aligned nanofiber scaffold used in this study constitutes a promising base material for tissue-engineered ligament in that it provides a more biomimetic structure, including the preferable mechanical environment.
