The effect of cyclin D2 shRNA on the proliferation and apoptosis of LP-1 cell.
- Author:
Cha GUO
1
;
Jian HOU
;
Yu-bao CHEN
;
Hui-yan JIN
;
Wei-jun FU
;
Zhen-gang YUAN
;
Dong-xing WANG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Proliferation; Cyclin D2; Cyclins; genetics; Humans; RNA Interference; RNA, Double-Stranded; RNA, Small Interfering; Transfection; Tumor Cells, Cultured
- From: Chinese Journal of Hematology 2006;27(10):666-669
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct cyclin D2 (CCND2) short hairpin RNA ( shRNA) plasmid for repressing the expression of CCND2 in human myeloma cell line LP-1,and to detect its effect on the proliferation and apoptosis of LP-1 cell.
METHODSA CCND2 shRNA model was constructed and cloned into plasmid pGensil-2, then the plasmid was transfected into LP-1 cell in vitro. The CCND2 expression cell proliferation, cell cycle and cell apoptosis of the transfected LP-1 cells were studied by RT-PCR, trypanosome staining, flow cytometry and annexin V assay.
RESULTSThe transfection efficiency of LP-1 cell was 34. 2%. In the transfected LP-1 cell CCND2 mRNA expression was reduced significantly, the cell growth was inhibited significantly and the cell cycle was partly arrested in G, phase. The apoptosis rate of the transfected LP-1 cell after 72 h was (25.7+/-4.8)%.
CONCLUSIONThe inhibition of CCND2 in LP-1 cells could inhibit the cell growth and induce cell apoptosis. CCND2 maybe a new therapeutic target.
