Biodistribution study of 131I-gM-CSF in SCID mice bearing human leukemia.
- Author:
Yi-zhi XU
1
;
Shi-feng LOU
;
Yang-jia DENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Female; Flow Cytometry; Granulocyte-Macrophage Colony-Stimulating Factor; pharmacokinetics; HL-60 Cells; Humans; Iodine Radioisotopes; Leukemia, Myeloid, Acute; metabolism; Mice; Mice, SCID; Xenograft Model Antitumor Assays
- From: Chinese Journal of Hematology 2006;27(10):678-681
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the biodistribution of 131I-GM-CSF in SCID mice bearing human AML in vivo.
METHODSThe xenograft model of human leukemia was established in SCID mice. In the leukemia mice, the biodistribution of 131I-GM-CSF produced by chlo amine-T method was studied.
RESULTS(1)The inoculated HL-60 cells could grow in SCID mice, which developed leukemia after 4 weeks. (2) 131 I-GM-CSF was concentrated in spleen, bone marrow and tumor tissue of the mice. In spleen and bone marrow, 131 I-GM-CSF was uptaken to peak in 30 minutes after injection, the up taking rate was (442. 9+/-86. 4) % ID/g and (4283. 8+/-252. 8)% ID/g, respectively, and maintained on higher level in 24 hours. The injection of 131I resulted in an even distribution in the whole body.
CONCLUSIONS131 I-GM-CSF is able to concentrate electively in spleen, bone marrow and organs infiltrated by leukemia cells. The biodistribution of 131I-GM-CSF in the leukemia mice is tissue specific.
