Identification of fetal nucleated erythrocytes in maternal blood using short tandem repeat typing after primer extension preamplification.
- Author:
Hong-ying WEI
1
;
Gui-fang LONG
;
Wei-xiong LIN
;
Shu-quan LI
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Erythroblasts; Erythrocyte Count; Female; Fetus; cytology; Humans; Male; Microsatellite Repeats; Oligonucleotide Array Sequence Analysis; Pregnancy; Prenatal Diagnosis; methods; beta-Thalassemia; diagnosis
- From: Chinese Journal of Hematology 2006;27(10):687-689
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a method for identifying fetal nucleated erythrocytes (NRBCs) in maternal blood.
METHODSNRBCs in maternal blood were detected by benzidine staining and collected by micromanipulation. After primer extension preamplification (PEP) of the entire genome from a single NRBC, short tandem repeat (STR) genotype was analysed after further amplification of this gene. Single NRBC was differentiated as fetal or maternal origin by comparison of STR genotype of NRBC with its corresponding parents.
RESULTSNRBCs were found in all of 28 pregnant women in a range of 4 to 13 per 5 ml venous blood. About 43. 6% of NRBCs were determined to be fetal origin by STR typing.
CONCLUSIONThis method provides effective identification of fetal NRBCs and allows non-invasive prenatal genetic diagnosis using single fetal NRBC.
