Establishment of a selective inactivation adenosine A2A receptors mice model.
- Author:
Jian-Hong AN
1
;
Wei LI
;
Song XIE
;
Pei-Fang ZHU
;
Shuang-Shuang DAI
;
Ren-Ping XIONG
;
Chun HU
;
Yuanguo ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Gene Deletion; Mice; Mice, Inbred C57BL; Mice, Knockout; Models, Animal; Receptor, Adenosine A2A; genetics
- From: Chinese Journal of Hematology 2007;28(3):174-177
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a mice model with selective inactivation adenosine A2A receptors (A2ARs) in peripheral white blood cells (PWBC).
METHODSA2ARs were selectively inactivated in PWBCs by transplanting bone marrow cells (BMCs) from A2AR knockout (KO) mice into their wild type (WT) littermates after a single total body irradiation of 9.5 Gy or fractionated total body irradiation of 6.2 Gy x 2. The efficiency of reconstitution of bone marrow-derived cells in chimeric mice was assessed.
RESULTSPCR band patterns changed from the recipient pattern (one band of 330 bp) to the donor (two bands of 300 and 330 bp) pattern. Immunohistochemistry analysis showed that 10.21% of cells were A2AR+ in PWBCs in KO--> WT mice, whereas 96.72% of cells were A2AR+ in WT mice. The survival rates of mice irradiated with 6.2 Gy x 2 and transplanted with more than 6 x 10(6) BMCs were about 91%.
CONCLUSIONA murine model of selective inactivation adenosine A2A receptors in PWBCs was established successfully.
