Alterations of CD4+ CD25+ regulatory T cells in patients with idiopathic thrombocytopenic purpura.
- Author:
Yun LING
1
;
Xiang-Shan CAO
;
Zi-Qiang YU
;
Guang-Hua LUO
;
Xia BAI
;
Jian SU
;
Lan DAI
;
Chang-Geng RUAN
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Female; Forkhead Transcription Factors; metabolism; Humans; Interleukin-2 Receptor alpha Subunit; metabolism; Male; Middle Aged; Purpura, Thrombocytopenic, Idiopathic; immunology; metabolism; RNA, Messenger; metabolism; T-Lymphocytes, Regulatory; immunology
- From: Chinese Journal of Hematology 2007;28(3):184-188
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of CD4+ CD25+ regulatory T (Treg) cells in patients with ITP.
METHODSFlow cytometry was used to examine the number of CD4+ CD25+, CD4+ CD25 high, CD4+ Foxp3+ and CD4+ CD25+ Foxp3+ T cells. The level of Foxp3 mRNA expression was analyzed by realtime quantitative reverse transcriptase polymerase chain reaction (RT-PCR) . CD4+ CD25 high T cells was cocultured with CD4+ CD25 - T cells from patients or controls for assessing the regulatory properties of CD4+ CD25 Treg cells.
RESULTSThe proportion of CD4+ CD25+ T cells in the peripheral blood of patients with ITP [(15.64 +/- 5.82) %] was significantly higher than that in normal control group [(9.30 +/- 3.95)%] (P <0.01). There was no significant difference in the percentages of CD4+ CD25 high T cells between ITP patients and controls [(1.53 +/- 0.66)% versus (1.36 +/- 0.55)% (P = 0.317)]. But the number of CD4 Foxp 3+ T cells and CD4 + CD25+ Foxp3+ T cells in patients were significantly lower than that in control group (P <0.01). The expression of Foxp3 mRNA reduced (P < 0.01) and the suppressive activity of CD4+ CD25 high T cells is lower than that of healthy controls (P <0.01).
CONCLUSIONIn patients with ITP, both the number and immuno-regulative function of CD4+ CD25+ Treg cells are reduced.
