Cytogenetic and molecular analysis of 1193 cases with chronic myeloid leukemia.
- Author:
Dao-zi JIANG
1
;
Zhi-mei CHEN
;
Ji-yu LOU
;
Yun-gui WANG
;
Yi LIANG
;
Jie JIN
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Child; Child, Preschool; Female; Fusion Proteins, bcr-abl; genetics; Humans; Karyotyping; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; genetics; Male; Middle Aged; Retrospective Studies
- From: Chinese Journal of Hematology 2007;28(1):1-5
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the cytogenetic and molecular genetic features of chronic myeloid leukemia (CML) in Chinese.
METHODSA total of 1193 CML patients were retrospectively studied. Chromosome preparation of bone marrow cells was made using direct and short-term culture. Karyotype and bcr-abl fusion genes were analyzed by R-banding, RT-PCR, respectively.
RESULTSIn the 1193 cases, 98.07% was Ph chromosome positive (Ph+) and 1.93% negative (Ph-). In the Ph+ patients, 95.64% was classical Ph and 4.36% variant rearrangements. Additional genetic changes were demonstrated in 11.88% of classical Ph cases. Cytogenetic clonal evolution was found in 7.94% of patients in chronic phase (CP), 27.78% in accelerated phase (AP), and 49. 04% in blast crisis (BC). Among the classical Ph cases, +Ph, +8, -21 were found in 14.62%, 10.77% and 7.69% of them respectively. In patients in BC and AP, the most common additional chromosome changes were + Ph (28.57%), +8 (16.67%) and +19 (7.14%), while in CP, -21 (10.26%), +Ph (8.97%), and +8 (8.97%). The combination of +Ph and +8 (3.60%) was the most frequent of combination pattern. 524 cases were investigated for bcr-abl fusion gene, and 54.01% was b3a2 (+) and 27.67% b2a2 (+).
CONCLUSIONIn Chinese CML patients seem to have their unique features in terms of cytogenetic clonal evaluation.
