Detection of cytogenetic abnormalities involving chromosomes 5,7 and 8 in myelodysplastic syndromes with fluorescence in situ hybridization and its clinical significance.
- Author:
Yu CAI
1
;
You-wen QIN
;
Chun WANG
;
Juan YANG
;
Shi-ke YAN
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Aged, 80 and over; Chromosome Aberrations; Chromosomes, Human, Pair 5; genetics; Chromosomes, Human, Pair 7; genetics; Chromosomes, Human, Pair 8; genetics; Cytogenetic Analysis; Female; Humans; In Situ Hybridization, Fluorescence; Karyotyping; Male; Middle Aged; Myelodysplastic Syndromes; genetics
- From: Chinese Journal of Hematology 2007;28(1):6-10
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify the abnormal karyotypes by fluorescence in situ hybridization (FISH) and explore prognostic implications in patients with myelodysplastic syndromes (MDS).
METHODSFISH was used to detect the frequently occurring chromosome abnormalities (-5/5q, +8, -7/7q-) in 37 MDS cases. SPSS 11.5 software and correlation analysis were used to analyze the relativity among the abnormal chromosomes, the prognosis and the disease conversion in 37 MDS patients.
RESULTSKaryotype abnormalities were found in 21 (56.8%) of 37 cases, among which 6 (16.2%) were complex karyotypes, 9 (24.3%) +8, 2(5.4%) -5/5q-, 2(5.4%) -7/7q-. In the median time of follow-up of 12 months, 12 cases transformed into acute leukemia. Complex karyotypes were significantly associated with the poor prognosis and leukemia transformation. + 8 and -7/7q- abnormalities were correlated with the death.
CONCLUSIONSFISH was more sensitive than conventional cytogenetics for detecting mini-clonal abnormality. There are some differences in abnormal karyotypes between patients in China and the western countries. Multi-probes used in cytogenetic detections may predict the patient' s prognosis more accurately. The higher proportion of abnormal karyotypes the poorer prognosis.
